Emerging mass spectrometry techniques for the direct analysis of microbial colonies
نویسندگان
چکیده
منابع مشابه
Laser desorption mass spectrometry for microbial DNA analysis.
Recently, we demonstrated that a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) can be used to determine the molecular weight of polymerase chain reaction (PCR) products of intact 16S rRNA regions and to profile their restriction digests. This is the first time that MALDI-TOF MS with ultraviolet (UV) photoionization has been used to analyze a PCR pro...
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MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis
Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process i...
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چکیده ندارد.
Mass Spectrometry Imaging of Complex Microbial Communities
In the two decades since mass spectrometry imaging (MSI) was first applied to visualize the distribution of peptides across biological tissues and cells, the technique has become increasingly effective and reliable. MSI excels at providing complementary information to existing methods for molecular analysis-such as genomics, transcriptomics, and metabolomics-and stands apart from other chemical...
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ژورنال
عنوان ژورنال: Current Opinion in Microbiology
سال: 2014
ISSN: 1369-5274
DOI: 10.1016/j.mib.2014.06.014